ABSTRACT
@#Duchene muscular dystrophy (DMD) is a serious progressive muscular dystrophy.Reports in recent years about abnormal lipid in DMD patients have increased, yet little attention has been paid to liver lipid.This study aimed to explore the effect of dystrophin gene defect on liver lipid synthesis.7-week-old mdx male mice were used as DMD model.The conditions of liver function, liver lipid accumulation and liver lipid synthesis were determined through liver tissue morphological examination, blood biochemical examination, and detection of hepatic gene and protein expression.The results showed that lipid droplets in liver of mdx mice increased significantly.The contents of total cholesterol and triglyceride in liver, aspartate aminotransferase and alanine aminotransferase in serum increased.The gene and protein expression of hepatic lipid synthesis-related enzymes such as fatty acid synthase, acetyl CoA carboxylase, and sterol regulatory element binding protein 1-c were up-regulated.These results showed accumulation of liver lipid in 7-week-old mdx male mice.
ABSTRACT
The“two-hit”theory which underlies the mechanism of non-alcoholic fatty liver disease (NAFLD) is difficult to explain the progress of NAFLD from liver lipid accumulation to steatohepatitis, liver fibrosis and the gradually aggravated diseases.Recently the“multiple-hit”theory emphasizes the significance of endoplasmic reticulum stress (ERS) in the progression of NAFLD. The endoplasmic reticulum is a cytoplasmic organella for protein and lipid synthesis. ERS, which can be induced by the unbalance of endoplasmic reticulum homeostasis, plays a critical role in hepatocyte lipid metabolic disorders, Apoptosis and the progression of NAFLD. In this paper, the relevance of ERS and non-alcoholic liver lipid accumulation, steatohepatitis and hepatocyte apoptosis is reviewed.